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KMID : 1157520170130010005
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International Journal of Clinical Preventive Dentistry
2017 Volume.13 No. 1 p.5 ~ p.10
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Investigation of Efficacy of New Electrolyzed Water Generator for Oral Bacteria
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Cho In-Woo
Nam Tae-Kye
Baek Dong-Heon
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Abstract
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Objective: Electrolyzed water has antimicrobial activity against pathogenic bacteria. However, antimicrobial efficacy of electrolyzed water (EW) generator varies depending on the configuration and for of the electrodes. This study is to investigate antibacterial activity of new electrolyzed water generator against oral bacteria and biofilm.
Methods: The EW was generated with eBio-cleaner, and the EW was treated on bacterial pellets after harvest of four oral
bacteria. The EW treated bacteria were spread on agar plate and incubated at 37°C in anaerobic condition. Next, the salivary biofilm was formed using pooled saliva from the healthy donor. The salivary biofilm was treated with tap water, the EW and listerine, and the biofilm bacteria were spread on agar plate and were observed confocal laser microscope after live/dead staining.
Results: The EW generated by eBio-cleaner has bactericidal activity against planktonic Aggregatibacter actinomycetemcomitans, Fusobacterium nucleatum, Porphyromonas gingivalis, and Streptococcus mutans. Listerine showed the strongest antimicrobial activity against planktonic bacteria. In case of biofilm experiment, the EW generated by eBio-cleaner showed disruptive and antimicrobial effect on the salivary biofilm. Whereas, listerine exhibited antimicrobial effect on the outside of biofilm.
Conclusion: Take together of the results, the EW generated by eBio-cleaner may be helpful in preventing and treating dental caries and periodontitis by oral bacteria.
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KEYWORD
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electrolyzed water generator, antimicrobial activity, oral biofilm, periodontopathogens, cariogenic bacteria
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